Determination of HER2 Gene Amplification in Breast Cancer using Dual-color Silver Enhanced in situ Hybridization (dc-SISH) and Comparison with Fluorescence ISH (FISH)
Copyright policy )Determination of HER2 Gene Amplification in Breast Cancer using Dual-color Silver Enhanced in situ Hybridization (dc-SISH) and Comparison with Fluorescence ISH (FISH)
The two basic methods that are currently accepted to identify the HER2 status are immunohistochemistry and flyorescence in situ hybridization (FISH) . The aim of this study was to perform the dual-color silver in situ hybridization (dc-SISH) technique as an alternative to FISH.A total of 40 invasive breast carcinoma cases were assessed for HER2 gene amplification by FISH and dual- color SISH.Significant correlation was found in the HER2 expression results obtained with the two approaches (p=0.001, p<0.05). The concordance rate was 92.3%.Foutine practical use of the dc-SISH method, which is much easier to apply, score, and evaluate, has many advantages. HER2 and CEN17 status can be evaluated simultaneously with the newly developed "Dual-Color Probe". All these specifications and the reliable results obtained support the widespread use of SISH technique in clinical practice.
Silver Staining, Receptor, ErbB-2, Gene Amplification, Breast Neoplasms, Prognosis, Biomarkers, Tumor, Humans, Female, Neoplasm Invasiveness, In Situ Hybridization, In Situ Hybridization, Fluorescence, Neoplasm Staging
Silver Staining, Receptor, ErbB-2, Gene Amplification, Breast Neoplasms, Prognosis, Biomarkers, Tumor, Humans, Female, Neoplasm Invasiveness, In Situ Hybridization, In Situ Hybridization, Fluorescence, Neoplasm Staging
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