Determinants of the nucleolar targeting of protein phosphatase‐1
Copyright policy )Determinants of the nucleolar targeting of protein phosphatase‐1
The ubiquitously expressed protein Ser/Thr phosphatase‐1 isoforms PP1α, PP1β and PP1γ1 are dynamically targeted to distinct, but overlapping cellular compartments by associated proteins. Within the nucleus of HeLa cells, EGFP‐tagged PP1γ1 and PP1β were predominantly targeted to the nucleoli, while PP1α showed a more diffuse distribution. Using PP1 chimaeras and point mutants we show here that a single N‐terminal residue, i.e., Gln20 for PP1α, Arg19 for PP1β and Arg20 for PP1γ1 accounts for their distinct subnuclear distribution. Our data also suggest that the N‐terminus of PP1β and PP1γ1 harbours an interaction site for one or more nucleolar interactors.
- Katholieke Universiteit Leuven Belgium
Targeting, Protein Conformation, Recombinant Fusion Proteins, Green Fluorescent Proteins, Molecular Sequence Data, Protein phosphatase-1, Nucleolus, Dephosphorylation, Isoenzymes, Protein Transport, Protein Phosphatase 1, Phosphoprotein Phosphatases, Humans, Point Mutation, Amino Acid Sequence, Cell Nucleolus, HeLa Cells
Targeting, Protein Conformation, Recombinant Fusion Proteins, Green Fluorescent Proteins, Molecular Sequence Data, Protein phosphatase-1, Nucleolus, Dephosphorylation, Isoenzymes, Protein Transport, Protein Phosphatase 1, Phosphoprotein Phosphatases, Humans, Point Mutation, Amino Acid Sequence, Cell Nucleolus, HeLa Cells
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