Identification of the fourth member of the mammalian endoprotease family homologous to the yeast Kex2 protease. Its testis-specific expression.
Copyright policy )Identification of the fourth member of the mammalian endoprotease family homologous to the yeast Kex2 protease. Its testis-specific expression.
We used the polymerase chain reaction to identify a mouse testis cDNA that represented another member of a growing class of mammalian endoproteases involved in the processing of precursor proteins. This cDNA encoded a 655-residue protein, designated PC4, containing a bacterial subtilisin-like catalytic domain closely related to those of the recently characterized precursor-processing endoproteases, furin, PC1/PC3, PC2, and Kex2. Within this domain, the amino acid sequence of PC4 was 70, 58, 55, and 45% identical with those of mouse furin, mouse PC1/PC3, mouse PC2, and yeast Kex2, respectively. Northern blot analysis indicated that the PC4 mRNA was detectable only in the testes after the 20th day of postnatal development. Moreover, this message was mainly expressed in the round spermatids. These data suggest that PC4 represents a prime candidate for a precursor-processing endoprotease in the testicular germ cells and that its gene expression is regulated during spermatogenesis.
- University of Tsukuba Japan
Male, Genome, Base Sequence, Genes, Fungal, Molecular Sequence Data, Ovary, Mice, Inbred Strains, DNA, Polymerase Chain Reaction, Epithelium, Mice, Oligodeoxyribonucleotides, Organ Specificity, Multigene Family, Endopeptidases, Animals, Female, Amino Acid Sequence, Proprotein Convertases, Cloning, Molecular
Male, Genome, Base Sequence, Genes, Fungal, Molecular Sequence Data, Ovary, Mice, Inbred Strains, DNA, Polymerase Chain Reaction, Epithelium, Mice, Oligodeoxyribonucleotides, Organ Specificity, Multigene Family, Endopeptidases, Animals, Female, Amino Acid Sequence, Proprotein Convertases, Cloning, Molecular
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).283 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 0.1%
Citations provided by BIP!Popularity provided by BIP!