Copyright information:Taken from "The GOLD domain, a novel protein module involved in Golgi function and secretion"Genome Biology 2002;3(5):research0023.1-research0023.7.Published online 24 Apr 2002PMCID:PMC115225.Copyright © 2002 Anantharaman and Aravind, licensee BioMed Central Ltd The PHD-secondary structure [15] is shown above the alignment, with E representing a β strand (upper-case letters indicate predictions with > 82% accuracy, and lower-case letters denote predictions with > 72% accuracy). A search with the lumenal region of p24-family member K08E4.6 (region 20-191) recovers RALBP from , the squid ortholog of SEC14L2 ( = 7 × 10, iteration 1). A reciprocal search with RALBP_ (region 189-343) recovers GCP60 ( = 2 × 10, iteration 1), FYCO1 (8 × 10, iteration 1), SPAC23H4.01c (10, iteration 2), KIAA0420 (10, iteration 2) and K08E4.6 (7 × 10, iteration 2). The 80% consensus shown below the alignment was derived using the following amino-acid classes: h, hydrophobic (ALICVMYFW, yellow shading); l, the aliphatic subset of the hydrophobic class (ALIVMC, yellow shading); a, aromatic (FHWY, yellow shading); s, small (ACDGNPSTV, green letters); u and p are the tiny subsets of the small class (u, GAS, green shading) and polar (p, CDEHKNQRST, blue letters). Y denotes a conserved tyrosine residue. The limits of the domains are indicated by the residue positions on each side. The numbers within the alignment indicate poorly conserved inserts that are not shown. The different families are shown on the right. A, p24 family; B, Osh3p family; C, CG9528 family (Sec14-like proteins with an amino-terminal PRELI/MSF1p' domain); D, Sec14-like proteins; E,GCP60 family; and F, FYCO1. The sequences are denoted by their gene name followed by the species abbreviation and GenBank Identifier. Ce, ; Dm, ; Hs, ; Sc, ; Sp, ; Top, .