PI(3,4,5)P3 Interactome
Copyright policy )PI(3,4,5)P3 Interactome
Immobilizing chemically synthesized analogues of PI(3,4,5)P3 onto Affi-10 beads and incorporating them into liposomes allowed their use as affinity absorbents in the comprehensive analysis of the phosphoinositide interactome using cytosolic cell extracts of the LIM1215 colon cancer cell line. This led to the identification of 282 proteins that either interact with PI(3,4,5)P3 or are indirectly captured as part of a complex containing a PI(3,4,5)P3 binding partner. Identification of the proteins was achieved using affinity/LC-MS/MS experiments.
- University of Melbourne Australia
- Ludwig Cancer Research Australia
- Ludwig Cancer Research Belgium
- Melbourne Health Australia
- Royal Melbourne Hospital Australia
Proteomics, Proteome, Carcinoma, Computational Biology, Mass Spectrometry, Phosphates, Cytosol, Models, Chemical, Phosphatidylinositol Phosphates, Cell Line, Tumor, Colonic Neoplasms, Liposomes, Protein Interaction Mapping, Humans, Glutathione Transferase
Proteomics, Proteome, Carcinoma, Computational Biology, Mass Spectrometry, Phosphates, Cytosol, Models, Chemical, Phosphatidylinositol Phosphates, Cell Line, Tumor, Colonic Neoplasms, Liposomes, Protein Interaction Mapping, Humans, Glutathione Transferase
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