Synaptic Vesicle Size and Number Are Regulated by a Clathrin Adaptor Protein Required for Endocytosis
Copyright policy )Synaptic Vesicle Size and Number Are Regulated by a Clathrin Adaptor Protein Required for Endocytosis
Clathrin-mediated endocytosis is thought to involve the activity of the clathrin adaptor protein AP180. However, the role of this protein in endocytosis in vivo remains unknown. Here, we show that a mutation that eliminates an AP180 homolog (LAP) in Drosophila severely impairs the efficiency of synaptic vesicle endocytosis and alters the normal localization of clathrin in nerve terminals. Most importantly, the size of both synaptic vesicles and quanta is significantly increased in lap mutants. These results provide novel insights into the molecular mechanism of endocytosis and reveal a role for AP180 in regulating vesicle size through a clathrin-dependent reassembly process.
- University of Wisconsin–Oshkosh United States
- Baylor College of Medicine United States
- University of Massachusetts System United States
- University of Massachusetts Amherst United States
- Howard Hughes Medical Institute United States
Nerve Endings, Embryo, Nonmammalian, Neuroscience(all), Molecular Sequence Data, Gene Expression Regulation, Developmental, Nuclear Proteins, Nerve Tissue Proteins, Phosphoproteins, Axons, Endocytosis, DNA-Binding Proteins, Adaptor Proteins, Vesicular Transport, Monomeric Clathrin Assembly Proteins, CCAAT-Enhancer-Binding Proteins, Animals, Drosophila, Amino Acid Sequence, Synaptic Vesicles, Sequence Alignment, Gene Library
Nerve Endings, Embryo, Nonmammalian, Neuroscience(all), Molecular Sequence Data, Gene Expression Regulation, Developmental, Nuclear Proteins, Nerve Tissue Proteins, Phosphoproteins, Axons, Endocytosis, DNA-Binding Proteins, Adaptor Proteins, Vesicular Transport, Monomeric Clathrin Assembly Proteins, CCAAT-Enhancer-Binding Proteins, Animals, Drosophila, Amino Acid Sequence, Synaptic Vesicles, Sequence Alignment, Gene Library
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