Proteomic and functional analysis of Argonaute‐containing mRNA–protein complexes in human cells
Copyright policy )Proteomic and functional analysis of Argonaute‐containing mRNA–protein complexes in human cells
Members of the Argonaute (Ago) protein family associate with small RNAs and have important roles in RNA silencing. Here, we analysed Ago1- and Ago2-containing protein complexes in human cells. Separation of Ago-associated messenger ribonucleoproteins (mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA-induced silencing complex activities. A comprehensive proteomic analysis of Ago-containing mRNPs identified a large number of proteins involved in RNA metabolism. By using co-immunoprecipitation experiments followed by RNase treatment, we biochemically mapped interactions within Ago mRNPs. Using reporter assays and knockdown experiments, we showed that the putative RNA-binding protein RBM4 is required for microRNA-guided gene regulation.
- Max Planck Society Germany
- University of Queensland Australia
- University of Queensland Australia
- Max Planck Institute of Biochemistry Germany
Ribonuclease III, microRNA, Proteome, Macromolecular Substances, Eukaryotic Initiation Factor-2, Gene silencing, RNA-Binding Proteins, Carboxypeptidases, Cell Line, Proto-Oncogene Proteins p21(ras), Argonaute proteins, MicroRNAs, RNA interference, Ribonucleoproteins, Genes, Reporter, Proto-Oncogene Proteins, Argonaute Proteins, ras Proteins, Humans, Gene Silencing, RNA, Messenger, Eukaryotic Initiation Factors
Ribonuclease III, microRNA, Proteome, Macromolecular Substances, Eukaryotic Initiation Factor-2, Gene silencing, RNA-Binding Proteins, Carboxypeptidases, Cell Line, Proto-Oncogene Proteins p21(ras), Argonaute proteins, MicroRNAs, RNA interference, Ribonucleoproteins, Genes, Reporter, Proto-Oncogene Proteins, Argonaute Proteins, ras Proteins, Humans, Gene Silencing, RNA, Messenger, Eukaryotic Initiation Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).306 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!