DIGE-based proteomic analysis identifies nucleophosmin/B23 and nucleolin C23 as over-expressed proteins in relapsed/refractory acute leukemia
Copyright policy )DIGE-based proteomic analysis identifies nucleophosmin/B23 and nucleolin C23 as over-expressed proteins in relapsed/refractory acute leukemia
Drug resistance is a challenge in treatment of acute leukemia. To investigate novel protein changes involved in resistance, protein expression profiles between leukemia cell line HL-60 and adriamycin-resistant HL-60 (HL-60/ADR) were compared based on a proteomic approach-2D-DIGE followed by MALDI-TOF/MS. 13 protein spots were identified as up-regulated and 3 down-regulated in HL-60/ADR. Nucleophosmin/B23 (NPM B23) and nucleolin C23 (C23) were selected and verified by western blot, which showed an obvious up-regulation in leukemia cells, especially in 3 resistant leukemia cell lines and in relapsed/refractory patients. To a conclusion, B23 and C23 may be involved in drug resistance and be useful in assessing the prognosis of leukemia.
- Fujian Medical University China (People's Republic of)
- Union Hospital China (People's Republic of)
Adult, Male, Proteomics, Leukemia, Adolescent, Blotting, Western, Nuclear Proteins, Middle Aged, Phosphoproteins, Survival Rate, Treatment Outcome, Drug Resistance, Neoplasm, Case-Control Studies, Acute Disease, Tumor Cells, Cultured, Humans, Female, Neoplasm Recurrence, Local, Nucleophosmin, Aged
Adult, Male, Proteomics, Leukemia, Adolescent, Blotting, Western, Nuclear Proteins, Middle Aged, Phosphoproteins, Survival Rate, Treatment Outcome, Drug Resistance, Neoplasm, Case-Control Studies, Acute Disease, Tumor Cells, Cultured, Humans, Female, Neoplasm Recurrence, Local, Nucleophosmin, Aged
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