Rac1 and Cdc42 Capture Microtubules through IQGAP1 and CLIP-170
Copyright policy )Rac1 and Cdc42 Capture Microtubules through IQGAP1 and CLIP-170
Linkage of microtubules to special cortical regions is essential for cell polarization. CLIP-170 binds to the growing ends of microtubules and plays pivotal roles in orientation. We have found that IQGAP1, an effector of Rac1 and Cdc42, interacts with CLIP-170. In Vero fibroblasts, IQGAP1 localizes at the polarized leading edge. Expression of carboxy-terminal fragment of IQGAP1, which includes the CLIP-170 binding region, delocalizes GFP-CLIP-170 from the tips of microtubules and alters the microtubule array. Activated Rac1/Cdc42, IQGAP1, and CLIP-170 form a tripartite complex. Furthermore, expression of an IQGAP1 mutant defective in Rac1/Cdc42 binding induces multiple leading edges. These results indicate that Rac1/Cdc42 marks special cortical spots where the IQGAP1 and CLIP-170 complex is targeted, leading to a polarized microtubule array and cell polarization.
rac1 GTP-Binding Protein, Biochemistry, Genetics and Molecular Biology(all), Macromolecular Substances, Cell Polarity, Microtubules, Actins, Neoplasm Proteins, Microscopy, Fluorescence, ras GTPase-Activating Proteins, COS Cells, Chlorocebus aethiops, Animals, Cattle, Pseudopodia, Carrier Proteins, cdc42 GTP-Binding Protein, Microtubule-Associated Proteins, Vero Cells, Cell Size, Protein Binding
rac1 GTP-Binding Protein, Biochemistry, Genetics and Molecular Biology(all), Macromolecular Substances, Cell Polarity, Microtubules, Actins, Neoplasm Proteins, Microscopy, Fluorescence, ras GTPase-Activating Proteins, COS Cells, Chlorocebus aethiops, Animals, Cattle, Pseudopodia, Carrier Proteins, cdc42 GTP-Binding Protein, Microtubule-Associated Proteins, Vero Cells, Cell Size, Protein Binding
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