Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids in Schizosaccharomyces pombe
Copyright policy )Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids in Schizosaccharomyces pombe
The stm1+ (SPAC17C9.10) gene of Schizosaccharomyces pombe is closely related to genes encoding vacuolar PQ-loop proteins, Ypq1, Ypq2, and Ypq3, of Saccharomyces cerevisiae. When stm1+ fused with GFP was expressed in fission or budding yeast, Stm1-GFP localized at the vacuolar membrane. Isolated vacuolar membrane vesicles from S. cerevisiae cells overexpressing stm1+ exhibited stm1+-dependent arginine and lysine uptake activity. Exchange activity of arginine and histidine/arginine, as observed for Ypq2 of S. cerevisiae, was also detected in the vesicles expressing stm1+. The expression levels of stm1+ in S. pombe cells significantly affected the vacuolar contents of lysine, histidine, and arginine. These results suggest that Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids across the vacuolar membrane.
- Kyushu University Japan
- Ehime University Japan
Saccharomyces cerevisiae Proteins, Lysine, Genetic Complementation Test, Green Fluorescent Proteins, Biological Transport, Active, Membrane Proteins, Membrane Transport Proteins, Intracellular Membranes, Saccharomyces cerevisiae, Arginine, Schizosaccharomyces, Vacuoles, Schizosaccharomyces pombe Proteins
Saccharomyces cerevisiae Proteins, Lysine, Genetic Complementation Test, Green Fluorescent Proteins, Biological Transport, Active, Membrane Proteins, Membrane Transport Proteins, Intracellular Membranes, Saccharomyces cerevisiae, Arginine, Schizosaccharomyces, Vacuoles, Schizosaccharomyces pombe Proteins
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