The Arabidopsis GNOM ARF-GEF Mediates Endosomal Recycling, Auxin Transport, and Auxin-Dependent Plant Growth
Copyright policy )The Arabidopsis GNOM ARF-GEF Mediates Endosomal Recycling, Auxin Transport, and Auxin-Dependent Plant Growth
Exchange factors for ARF GTPases (ARF-GEFs) regulate vesicle trafficking in a variety of organisms. The Arabidopsis protein GNOM is a brefeldin A (BFA) sensitive ARF-GEF that is required for the proper polar localization of PIN1, a candidate transporter of the plant hormone auxin. Mutations in GNOM lead to developmental defects that resemble those caused by interfering with auxin transport. Both PIN1 localization and auxin transport are also sensitive to BFA. In this paper, we show that GNOM localizes to endosomes and is required for their structural integrity. We engineered a BFA-resistant version of GNOM. In plants harboring this fully functional GNOM variant, PIN1 localization and auxin transport are no longer sensitive to BFA, while trafficking of other proteins is still affected by the drug. Our results demonstrate that GNOM is required for the recycling of auxin transport components and suggest that ARF-GEFs regulate specific endosomal trafficking pathways.
- RIKEN Japan
- French National Centre for Scientific Research France
- Centre national de la recherche scientifique France
- University of Tübingen Germany
Brefeldin A, Microscopy, Confocal, Indoleacetic Acids, Biochemistry, Genetics and Molecular Biology(all), Arabidopsis Proteins, Molecular Sequence Data, Arabidopsis, Drug Resistance, Biological Transport, Active, Membrane Proteins, Membrane Transport Proteins, Endosomes, Plant Growth Regulators, Mutation, Guanine Nucleotide Exchange Factors, Amino Acid Sequence, Genetic Engineering
Brefeldin A, Microscopy, Confocal, Indoleacetic Acids, Biochemistry, Genetics and Molecular Biology(all), Arabidopsis Proteins, Molecular Sequence Data, Arabidopsis, Drug Resistance, Biological Transport, Active, Membrane Proteins, Membrane Transport Proteins, Endosomes, Plant Growth Regulators, Mutation, Guanine Nucleotide Exchange Factors, Amino Acid Sequence, Genetic Engineering
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