Tools for the study of genome rearrangements in laboratory and industrial yeast strains
Copyright policy )Tools for the study of genome rearrangements in laboratory and industrial yeast strains
AbstractIn order to investigate the extent of genome rearrangements in laboratory and industrial yeast strains, a set of plasmids, containing ca. 300 bp fragments from highly conserved genes from S. cerevisiae, has been constructed. We chose three unique PCR products, each from a single gene, per chromosome: one from close to the centromere, and one from each chromosome end. Using these plasmids as probes to hybridize a Southern blot from a pulsed‐field gel electrophoresis separation of the 16 yeast chromosomes, it is possible to identify large chromosomal rearrangements such as reciprocal translocations. Copyright © 2002 John Wiley & Sons, Ltd.
- University of Salford United Kingdom
Chromosome Aberrations, Evolution, Genome rearrangements, Saccharomyces cerevisiae, Polymerase Chain Reaction, Electrophoresis, Gel, Pulsed-Field, Evolution, Molecular, Blotting, Southern, ResearchInstitutes_Networks_Beacons/manchester_institute_of_biotechnology; name=Manchester Institute of Biotechnology, Manchester Institute of Biotechnology, Translocations, Karyotypes, Genome, Fungal, DNA Probes, DNA, Fungal, Plasmids
Chromosome Aberrations, Evolution, Genome rearrangements, Saccharomyces cerevisiae, Polymerase Chain Reaction, Electrophoresis, Gel, Pulsed-Field, Evolution, Molecular, Blotting, Southern, ResearchInstitutes_Networks_Beacons/manchester_institute_of_biotechnology; name=Manchester Institute of Biotechnology, Manchester Institute of Biotechnology, Translocations, Karyotypes, Genome, Fungal, DNA Probes, DNA, Fungal, Plasmids
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