Solution Structure of the B3 DNA Binding Domain of the Arabidopsis Cold-Responsive Transcription Factor RAV1[W]
Copyright policy )Solution Structure of the B3 DNA Binding Domain of the Arabidopsis Cold-Responsive Transcription Factor RAV1[W]
AbstractThe B3 DNA binding domain is shared amongst various plant-specific transcription factors, including factors involved in auxin-regulated and abscisic acid–regulated transcription. Herein, we report the NMR solution structure of the B3 domain of the Arabidopsis thaliana cold-responsive transcription factor RAV1. The structure consists of a seven-stranded open β-barrel and two α-helices located at the ends of the barrel and is significantly similar to the structure of the noncatalytic DNA binding domain of the restriction enzyme EcoRII. An NMR titration experiment revealed a DNA recognition interface that enabled us to propose a structural model of the protein–DNA complex. The locations of the DNA-contacting residues are also likely to be similar to those of the EcoRII DNA binding domain.
Models, Molecular, Binding Sites, Sequence Homology, Amino Acid, Arabidopsis Proteins, Acclimatization, Arabidopsis, Molecular Conformation, DNA, Protein Structure, Secondary, Protein Structure, Tertiary, Cold Temperature, DNA-Binding Proteins, Evolution, Molecular, Amino Acid Sequence, Deoxyribonucleases, Type II Site-Specific, Nuclear Magnetic Resonance, Biomolecular, Phylogeny
Models, Molecular, Binding Sites, Sequence Homology, Amino Acid, Arabidopsis Proteins, Acclimatization, Arabidopsis, Molecular Conformation, DNA, Protein Structure, Secondary, Protein Structure, Tertiary, Cold Temperature, DNA-Binding Proteins, Evolution, Molecular, Amino Acid Sequence, Deoxyribonucleases, Type II Site-Specific, Nuclear Magnetic Resonance, Biomolecular, Phylogeny
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).120 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!