Molecular Interactions Stabilizing the Promatrix Metalloprotease-9·Serglycin Heteromer
Copyright policy )Molecular Interactions Stabilizing the Promatrix Metalloprotease-9·Serglycin Heteromer
Previous studies have shown that THP-1 cells produced an SDS-stable and reduction-sensitive complex between proMMP-9 and a chondroitin sulfate proteoglycan (CSPG) core protein. The complex could be reconstituted in vitro using purified serglycin (SG) and proMMP-9 and contained no inter-disulfide bridges. It was suggested that the complex involved both the FnII module and HPX domain of proMMP-9. The aims of the present study were to resolve the interacting regions of the molecules that form the complex and the types of interactions involved. In order to study this, we expressed and purified full-length and deletion variants of proMMP-9, purified CSPG and SG, and performed in vitro reconstitution assays, peptide arrays, protein modelling, docking, and molecular dynamics (MD) simulations. ProMMP-9 variants lacking both the FnII module and the HPX domain did not form the proMMP-9∙CSPG/SG complex. Deletion variants containing at least the FnII module or the HPX domain formed the proMMP-9∙CSPG/SG complex, as did the SG core protein without CS chains. The interacting parts covered large surface areas of both molecules and implicated dynamic and complementary ionic, hydrophobic, and hydrogen bond interactions. Hence, no short single interacting linear motifs in the two macromolecules could explain the strong SDS-stable and reduction-sensitive binding.
Models, Molecular, in vitro reconstitution, Protein Conformation, THP-1 Cells, Vesicular Transport Proteins, Molecular Dynamics Simulation, proMMP-9 deletion variants, Article, Cell Line, Protein Domains, Sf9 Cells, Animals, Humans, proMMP-9 complexes, proMMP-9, Sequence Deletion, Binding Sites, VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710, Hydrogen Bonding, peptide arrays, VDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710, molecular modelling, Molecular Docking Simulation, molecular dynamics simulation, Matrix Metalloproteinase 9, docking, serglycin, Proteoglycans, Protein Binding
Models, Molecular, in vitro reconstitution, Protein Conformation, THP-1 Cells, Vesicular Transport Proteins, Molecular Dynamics Simulation, proMMP-9 deletion variants, Article, Cell Line, Protein Domains, Sf9 Cells, Animals, Humans, proMMP-9 complexes, proMMP-9, Sequence Deletion, Binding Sites, VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710, Hydrogen Bonding, peptide arrays, VDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710, molecular modelling, Molecular Docking Simulation, molecular dynamics simulation, Matrix Metalloproteinase 9, docking, serglycin, Proteoglycans, Protein Binding
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