Light-induced retinal injury enhanced neurotrophins secretion and neurotrophic effect of mesenchymal stem cells in vitro
Copyright policy )Light-induced retinal injury enhanced neurotrophins secretion and neurotrophic effect of mesenchymal stem cells in vitro
To investigate neurotrophins expression and neurotrophic effect change in mesenchymal stem cells (MSCs) under different types of stimulation.Rats were exposed in 10,000 lux white light to develop light-induced retinal injury. Supernatants of homogenized retina (SHR), either from normal or light-injured retina, were used to stimulate MSCs. Quantitative real time for polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were conducted for analysis the expression change in basic fibroblast growth factor (bFGF), brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) in MSCs after stimulation. Conditioned medium from SHR-stimulated MSCs and control MSCs were collected for evaluation their effect on retinal explants.Supernatants of homogenized retina from light-injured rats significantly promoted neurotrophins secretion from MSCs (p<0.01). Conditioned medium from mesenchymal stem cells stimulated by light-injured SHR significantly reduced DNA fragmentation (p<0.01), up-regulated bcl-2 (p<0.01) and down-regulated bax (p<0.01) in retinal explants, displaying enhanced protective effect.Light-induced retinal injury is able to enhance neurotrophins secretion from mesenchymal stem cells and promote the neurotrophic effect of mesenchymal stem cells.
- Fujian Medical University China (People's Republic of)
- First Affiliated Hospital of Fujian Medical University China (People's Republic of)
Ciliary neurotrophic factor, Light, Mesenchymal stromal cells, Stem cells, Real-Time Polymerase Chain Reaction, Brain-derived neurotrophic factor, Retina, Rats, Sprague-Dawley, Random Allocation, Animals, Ciliary Neurotrophic Factor, Nerve Growth Factors, Cells, Cultured, Fator neurotrófico ciliar, Células mesenquimais estromais, Brain-Derived Neurotrophic Factor, Fibroblast growth factor 2, Fator neurotrófico derivado do encéfalo, Mesenchymal Stem Cells, Retina/injuries, Rats, Fibroblast Growth Factors, Disease Models, Animal, Fator 2 de crescimento de fibroblastos, Células-tronco
Ciliary neurotrophic factor, Light, Mesenchymal stromal cells, Stem cells, Real-Time Polymerase Chain Reaction, Brain-derived neurotrophic factor, Retina, Rats, Sprague-Dawley, Random Allocation, Animals, Ciliary Neurotrophic Factor, Nerve Growth Factors, Cells, Cultured, Fator neurotrófico ciliar, Células mesenquimais estromais, Brain-Derived Neurotrophic Factor, Fibroblast growth factor 2, Fator neurotrófico derivado do encéfalo, Mesenchymal Stem Cells, Retina/injuries, Rats, Fibroblast Growth Factors, Disease Models, Animal, Fator 2 de crescimento de fibroblastos, Células-tronco
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