An SRY-related gene expressed during spermatogenesis in the mouse encodes a sequence-specific DNA-binding protein.
Copyright policy )An SRY-related gene expressed during spermatogenesis in the mouse encodes a sequence-specific DNA-binding protein.
SRY, the testis determining gene, encodes a member of a family of DNA binding proteins characterized by an amino acid sequence motif known as the HMG box. Using degenerate primers and the polymerase chain reaction, we have isolated SRY-related cDNAs from adult murine testis RNA. One of these, Sox-5, encodes a 43 kDa HMG-box protein with similarities to transcription activating proteins. Anti-Sox-5 antibody was used to analyse expression of Sox-5 in pre-pubertal testis and in fractionated spermatogenic cells. Sox-5 is restricted to post-meiotic germ cells, being found at highest levels in round spermatids. Sox-5 is a DNA binding protein and binding site selection assays suggest that it can bind specifically to oligonucleotides containing the consensus motif AACAAT. Sry can also bind to this motif, indicating that the Sry family may have overlapping sequence specificities.
- Institute of Cancer Research United Kingdom
Male, Base Sequence, Sequence Homology, Amino Acid, Immunoblotting, Molecular Sequence Data, High Mobility Group Proteins, Nuclear Proteins, Mice, Inbred Strains, DNA, Polymerase Chain Reaction, Cell Line, DNA-Binding Proteins, Mice, Oligodeoxyribonucleotides, Multigene Family, Animals, Humans, Amino Acid Sequence, RNA, Messenger, Gene Library
Male, Base Sequence, Sequence Homology, Amino Acid, Immunoblotting, Molecular Sequence Data, High Mobility Group Proteins, Nuclear Proteins, Mice, Inbred Strains, DNA, Polymerase Chain Reaction, Cell Line, DNA-Binding Proteins, Mice, Oligodeoxyribonucleotides, Multigene Family, Animals, Humans, Amino Acid Sequence, RNA, Messenger, Gene Library
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