The molecular cloning of a dispersed set of developmentally regulated genes which encode the major larval serum protein of D. melanogaster
Copyright policy )The molecular cloning of a dispersed set of developmentally regulated genes which encode the major larval serum protein of D. melanogaster
We have isolated chromosomal segments of D. melanogaster DNA that encode the alpha, beta and gamma polypeptides of the major larval serum protein (LSP1) from libraries of recombinant phage. The recombinant DNAs hybridize in situ to polytene chromosome regions 11A, 21D of 61A, consistent with the known cytogenetic positions of the alpha, beta and gamma genes, respectively. The three genes each encode a 2.85 kb polyadenylated RNA molecule. We have positioned these coding sequences, by both Southern hybridization analysis and R loop mapping. Heteroduplex analysis reveals comparatively close homology between the alpha and beta genes, less homology between the beta and gamma genes and least homology between the alpha and gamma genes.
- Imperial College London United Kingdom
Drosophila melanogaster, Base Sequence, Genes, Hemolymph, Larva, Nucleic Acid Heteroduplexes, Animals, Blood Proteins, Cloning, Molecular, Chromosomes
Drosophila melanogaster, Base Sequence, Genes, Hemolymph, Larva, Nucleic Acid Heteroduplexes, Animals, Blood Proteins, Cloning, Molecular, Chromosomes
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