Class C Vps Protein Complex Regulates Vacuolar SNARE Pairing and Is Required for Vesicle Docking/Fusion
Copyright policy )Class C Vps Protein Complex Regulates Vacuolar SNARE Pairing and Is Required for Vesicle Docking/Fusion
In yeast, the Class C Vps protein complex (C-Vps complex), composed of Vps11, Vps16, Vps18, and Vps33, functions in Golgi-to-vacuole protein transport. In this study, we characterized and purified this complex and identified its interaction with the syntaxin homolog Vam3. Vam3 pairs with the SNAP-25 homolog Vam7 and VAMP homolog Vti1 to form SNARE complexes during vesicle docking/fusion with the vacuole. The C-Vps complex does not bind to Vam3-Vti1-Vam7 paired SNARE complexes but instead binds to unpaired Vam3. Antibodies to a component of this complex inhibited in vitro vacuole-to-vacuole fusion. Furthermore, temperature-conditional mutations in the Class C VPS genes destabilized Vam3-Vti1-Vam7 pairing. Therefore, we propose that the C-Vps complex associates with unpaired (activated) Vam3 to mediate the assembly of trans-SNARE complexes during both vesicle docking/fusion and vacuole-to-vacuole fusion.
- University of Göttingen Germany
- University of California, San Diego United States
- University of California, San Diego United States
Saccharomyces cerevisiae Proteins, Synaptosomal-Associated Protein 25, Qa-SNARE Proteins, Cytoplasmic Vesicles, Vesicular Transport Proteins, Gene Expression, Golgi Apparatus, Membrane Proteins, Nerve Tissue Proteins, Cell Biology, Membrane Fusion, Fungal Proteins, Adaptor Proteins, Vesicular Transport, Protein Transport, Munc18 Proteins, Yeasts, Vacuoles, Escherichia coli, Carrier Proteins, SNARE Proteins, Molecular Biology
Saccharomyces cerevisiae Proteins, Synaptosomal-Associated Protein 25, Qa-SNARE Proteins, Cytoplasmic Vesicles, Vesicular Transport Proteins, Gene Expression, Golgi Apparatus, Membrane Proteins, Nerve Tissue Proteins, Cell Biology, Membrane Fusion, Fungal Proteins, Adaptor Proteins, Vesicular Transport, Protein Transport, Munc18 Proteins, Yeasts, Vacuoles, Escherichia coli, Carrier Proteins, SNARE Proteins, Molecular Biology
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