Inhibition of Polo kinase by BI2536 affects centriole separation duringDrosophilamale meiosis
Copyright policy )Inhibition of Polo kinase by BI2536 affects centriole separation duringDrosophilamale meiosis
Pharmacological inhibition of Drosophila Polo kinase with BI2536 has allowed us to re-examine the requirements for Polo during Drosophila male gametogenesis. BI2536-treated spermatocytes persisted in a pro-metaphase state without dividing and had condensed chromosomes that did not separate. Centrosomes failed to recruit γ-tubulin and centrosomin (Cnn) and were not associated with microtubule arrays that were abnormal and did not form proper bipolar spindles. Centrioles, which usually separate during the anaphase of the first meiosis, remained held together in a V-shaped configuration suggesting that Polo kinase regulates the proteolysis that breaks centriole linkage to ensure their disengagement. Despite these defects spermatid differentiation proceeds, leading to axoneme formation.
- University of Siena Italy
- University of Siena Italy
- University of Cambridge United Kingdom
- California Institute of Technology United States
centrosome maturation, Meiosi, Male, 570, BI2536; Centriole disengagement; Centrosome maturation; Drosophila; Gametogenesis; Polo kinase; Spindle assembly; Animals; Axoneme; Centrioles; Chromatids; Drosophila; Drosophila Proteins; Male; Meiosis; Protein-Serine-Threonine Kinases; Pteridines; Pupa; Spermatocytes; Tubulin; Cell Biology; Molecular Biology; Developmental Biology, Axoneme, Protein-Serine-Threonine Kinase, Chromatids, Protein Serine-Threonine Kinases, Centrosome maturation, gametogenesis, spindle assembly, Spermatocytes, Tubulin, Polo kinase, Animals, Drosophila Proteins, Molecular Biology, Centrioles, Animal, Chromatid, BI2536, Pteridines, Gametogenesi, Pupa, Centriole disengagement, Centriole, Cell Biology, Spermatocyte, Meiosis, centriole disengagement, Drosophila Protein, Pteridine, Drosophila, Spindle assembly, Developmental Biology
centrosome maturation, Meiosi, Male, 570, BI2536; Centriole disengagement; Centrosome maturation; Drosophila; Gametogenesis; Polo kinase; Spindle assembly; Animals; Axoneme; Centrioles; Chromatids; Drosophila; Drosophila Proteins; Male; Meiosis; Protein-Serine-Threonine Kinases; Pteridines; Pupa; Spermatocytes; Tubulin; Cell Biology; Molecular Biology; Developmental Biology, Axoneme, Protein-Serine-Threonine Kinase, Chromatids, Protein Serine-Threonine Kinases, Centrosome maturation, gametogenesis, spindle assembly, Spermatocytes, Tubulin, Polo kinase, Animals, Drosophila Proteins, Molecular Biology, Centrioles, Animal, Chromatid, BI2536, Pteridines, Gametogenesi, Pupa, Centriole disengagement, Centriole, Cell Biology, Spermatocyte, Meiosis, centriole disengagement, Drosophila Protein, Pteridine, Drosophila, Spindle assembly, Developmental Biology
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