Wnt-7a Up-regulates Matrix Metalloproteinase-12 Expression and Promotes Cell Proliferation in Corneal Epithelial Cells during Wound Healing
Copyright policy )Wnt-7a Up-regulates Matrix Metalloproteinase-12 Expression and Promotes Cell Proliferation in Corneal Epithelial Cells during Wound Healing
Corneal wound repair involves the rapid coverage of a denuded area by residual epithelial cells. During wound healing, there are different cell behaviors in different regions of the epithelium: cell proliferation in the peripheral epithelium and cell migration in the central epithelium. We found that Wnt-7a was rapidly induced in the wounded cornea, promoted the proliferation of corneal epithelial cells, and enhanced wound closure. Matrix metalloproteinase-12 (MMP-12) was detected in the peripheral epithelium, where cell proliferation was enhanced, but was diminished in the migrating central epithelium. Wnt-7a induced the accumulation of beta-catenin and the activation of Rac and beta-catenin, and Rac synergistically induced the transcription of MMP-12. Blocking the function of MMP-12 delayed wound closure induced by Wnt-7a. Our results also suggest that, in addition to the beta-catenin pathway, Wnt-7a might induce a beta-catenin-independent pathway. By regulating the proliferation of corneal epithelial cells, Wnt-7a and MMP-12 appear to contribute to corneal wound healing.
- Catholic University of Korea Korea (Republic of)
Chromatin Immunoprecipitation, Binding Sites, Blotting, Western, Cell Culture Techniques, Metalloendopeptidases, Epithelial Cells, Gene Expression Regulation, Enzymologic, Cell Line, Cornea, Cytoskeletal Proteins, Organ Culture Techniques, Cell Movement, Matrix Metalloproteinase 12, Proto-Oncogene Proteins, Animals, Humans, Luciferases, Cell Proliferation, Plasmids, Protein Binding
Chromatin Immunoprecipitation, Binding Sites, Blotting, Western, Cell Culture Techniques, Metalloendopeptidases, Epithelial Cells, Gene Expression Regulation, Enzymologic, Cell Line, Cornea, Cytoskeletal Proteins, Organ Culture Techniques, Cell Movement, Matrix Metalloproteinase 12, Proto-Oncogene Proteins, Animals, Humans, Luciferases, Cell Proliferation, Plasmids, Protein Binding
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