Prognostic relevance of LGALS3BP in human colorectal carcinoma
Copyright policy )Prognostic relevance of LGALS3BP in human colorectal carcinoma
A previous report has shown that LGALS3BP (also known as 90K or Mac-2 BP) has antitumor activity in colorectal cancer (CRC) via suppression of Wnt signalling with a novel mechanism of ISGylation-dependent ubiquitination of β-catenin. The role of LGALS3BP in CRC prognosis was investigated.The role of LGALS3BP on CRC progression and clinical prognosis was analyzed by combining cell cultures, in vitro assays, and immunohistochemistry.Silencing of LGALS3BP in HCT-116 human colon cancer cells resulted in enhanced β-catenin expression that was reversed by addition of human recombinant LGALS3BP. Moreover, intra-tumor delivery of LGALS3BP reduced tumor growth of xenografts originating from LGALS3BP-silenced HCT-116 cells. Finally, in a series of 196 CRC patients, LGALS3BP expression in tumor tissue associated with clinical outcome. Patients with high LGALS3BP expression had lower risk of relapse and a longer overall survival time than those with low LGALS3BP expression. Multivariate analyses confirmed LGALS3BP expression status as the only independent prognostic factor of survival.These results provide evidence that low expression of LGALS3BP participates in malignant progression of CRC and implicates poor prognosis, highlighting its augmentation as a potential therapeutic approach.
- University of Chieti-Pescara Italy
- Roma Tre University Italy
- Sapienza University of Rome Italy
Down-Regulation, Mice, Nude, Kaplan-Meier Estimate, Injections, Intralesional, Antigens, Neoplasm, Biomarkers, Tumor, Animals, Humans, Gene Silencing, Colorectal cancer; LGALS3BP; Prognosis; β-Catenin; Animals; Antigens, Neoplasm; Biomarkers, Tumor; Carrier Proteins; Cell Proliferation; Colorectal Neoplasms; Down-Regulation; Female; Gene Knockdown Techniques; Gene Silencing; Glycoproteins; HCT116 Cells; HEK293 Cells; Humans; Immunohistochemistry; Injections, Intralesional; Kaplan-Meier Estimate; Mice, Nude; Multivariate Analysis; Prognosis; Treatment Outcome; Xenograft Model Antitumor Assays; beta Catenin; Medicine (all); Biochemistry, Genetics and Molecular Biology (all), Cell Proliferation, Glycoproteins, Medicine(all), Biochemistry, Genetics and Molecular Biology(all), Research, Colorectal cancer; LGALS3BP; Prognosis; β-Catenin; Animals; Antigens, Neoplasm; Biomarkers, Tumor; Carrier Proteins; Cell Proliferation; Colorectal Neoplasms; Down-Regulation; Female; Gene Knockdown Techniques; Gene Silencing; Glycoproteins; HCT116 Cells; HEK293 Cells; Humans; Immunohistochemistry; Injections, Intralesional; Kaplan-Meier Estimate; Mice, Nude; Multivariate Analysis; Prognosis; Treatment Outcome; Xenograft Model Antitumor Assays; beta Catenin; Biochemistry, Genetics and Molecular Biology (all); Medicine (all), HCT116 Cells, Prognosis, Immunohistochemistry, HEK293 Cells, Gene Knockdown Techniques, Multivariate Analysis, Female, Carrier Proteins, Colorectal Neoplasms
Down-Regulation, Mice, Nude, Kaplan-Meier Estimate, Injections, Intralesional, Antigens, Neoplasm, Biomarkers, Tumor, Animals, Humans, Gene Silencing, Colorectal cancer; LGALS3BP; Prognosis; β-Catenin; Animals; Antigens, Neoplasm; Biomarkers, Tumor; Carrier Proteins; Cell Proliferation; Colorectal Neoplasms; Down-Regulation; Female; Gene Knockdown Techniques; Gene Silencing; Glycoproteins; HCT116 Cells; HEK293 Cells; Humans; Immunohistochemistry; Injections, Intralesional; Kaplan-Meier Estimate; Mice, Nude; Multivariate Analysis; Prognosis; Treatment Outcome; Xenograft Model Antitumor Assays; beta Catenin; Medicine (all); Biochemistry, Genetics and Molecular Biology (all), Cell Proliferation, Glycoproteins, Medicine(all), Biochemistry, Genetics and Molecular Biology(all), Research, Colorectal cancer; LGALS3BP; Prognosis; β-Catenin; Animals; Antigens, Neoplasm; Biomarkers, Tumor; Carrier Proteins; Cell Proliferation; Colorectal Neoplasms; Down-Regulation; Female; Gene Knockdown Techniques; Gene Silencing; Glycoproteins; HCT116 Cells; HEK293 Cells; Humans; Immunohistochemistry; Injections, Intralesional; Kaplan-Meier Estimate; Mice, Nude; Multivariate Analysis; Prognosis; Treatment Outcome; Xenograft Model Antitumor Assays; beta Catenin; Biochemistry, Genetics and Molecular Biology (all); Medicine (all), HCT116 Cells, Prognosis, Immunohistochemistry, HEK293 Cells, Gene Knockdown Techniques, Multivariate Analysis, Female, Carrier Proteins, Colorectal Neoplasms
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