Gene Regulation in Response to Overexpression of Cytochrome P450 and Proliferation of the Endoplasmic Reticulum inSaccharomyces cerevisiae
Copyright policy )Gene Regulation in Response to Overexpression of Cytochrome P450 and Proliferation of the Endoplasmic Reticulum inSaccharomyces cerevisiae
(CYP52A4) in Saccharomyces cerevisiae. Using the mRNA differential display technique, six genes were found to be up-regulated: ASN2, MDJ1, YLR194c, YNL208w, YER175, and YGL121c. Genes coding for Dur1.2p, Dal2p, and Sps19p were down-regulated. Two strongly induced genes, which were found to accommodate the peroxisome box (YLR194c) and a 10-bp consensus sequence of genes involved in lipid metabolism (YNL208w) in their promoter regions, were further analyzed with respect to the course of induction, the necessity of the P450 membrane anchor for induction, and the effects of gene disruption on P450Cm2 overexpression. We found that both genes are not essential to overproduce P450Cm2, but their induction was dependent on P450Cm2 membrane integration.
- Ludwig-Maximilians-Universität München Germany
- University of Tokyo Japan
- Friedrich Schiller University Jena Germany
- Drittes Physikalisches Institut Germany
Base Sequence, Gene Expression Profiling, Genes, Fungal, Saccharomyces cerevisiae, Endoplasmic Reticulum, Recombinant Proteins, Fungal Proteins, Kinetics, Cytochrome P-450 Enzyme System, Gene Expression Regulation, Fungal, Consensus Sequence, Peroxisomes, Sequence Deletion
Base Sequence, Gene Expression Profiling, Genes, Fungal, Saccharomyces cerevisiae, Endoplasmic Reticulum, Recombinant Proteins, Fungal Proteins, Kinetics, Cytochrome P-450 Enzyme System, Gene Expression Regulation, Fungal, Consensus Sequence, Peroxisomes, Sequence Deletion
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