CD4-Independent Binding of SIV gp120 to Rhesus CCR5
Copyright policy )CD4-Independent Binding of SIV gp120 to Rhesus CCR5
CCR5 and CD4 are coreceptors for immunodeficiency virus entry into target cells. The gp120 envelope glycoprotein from human immunodeficiency virus strain HIV-1(YU2) bound human CCR5 (CCR5 hu ) or rhesus macaque CCR5 (CCR5 rh ) only in the presence of CD4. The gp120 from simian immunodeficiency virus strain SIVmac239 bound CCR5 rh without CD4, but CCR5 hu remained CD4-dependent. The CD4-independent binding of SIVmac239 gp120 depended on a single amino acid, Asp 13 , in the CCR5 rh amino-terminus. Thus, CCR5-binding moieties on the immunodeficiency virus envelope glycoprotein can be generated by interaction with CD4 or by direct interaction with the CCR5 amino-terminus. These results may have implications for the evolution of receptor use among lentiviruses as well as utility in the development of effective intervention.
- Harvard University United States
- University of Padua Italy
- Tulane University United States
Membrane Glycoproteins, Receptors, CCR5, Macrophages, Antibodies, Monoclonal, HIV Antibodies, HIV Envelope Protein gp120, Transfection, Macaca mulatta, Cell Line, Viral Envelope Proteins, CD4 Antigens, HIV-2, Mutation, Animals, Humans, Simian Immunodeficiency Virus, Amino Acid Sequence
Membrane Glycoproteins, Receptors, CCR5, Macrophages, Antibodies, Monoclonal, HIV Antibodies, HIV Envelope Protein gp120, Transfection, Macaca mulatta, Cell Line, Viral Envelope Proteins, CD4 Antigens, HIV-2, Mutation, Animals, Humans, Simian Immunodeficiency Virus, Amino Acid Sequence
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