SMAR1, a Novel, Alternatively Spliced Gene Product, Binds the Scaffold/Matrix-Associated Region at the T Cell Receptor β Locus
Copyright policy )SMAR1, a Novel, Alternatively Spliced Gene Product, Binds the Scaffold/Matrix-Associated Region at the T Cell Receptor β Locus
Rearrangement and expression of the T cell receptor beta gene are critical events for early T lymphocyte development. To characterize cis-regulatory elements and their associated trans-factors that mediate these events, we have previously identified a nuclear matrix/scaffold-associated region, referred to as MARbeta, 400 bp upstream of the Ebeta enhancer. Electrophoretic mobility shift assay showed that two known MAR-binding proteins, SATB1 and Cux, bind MARbeta. In this article, we report the identification of a novel MAR-binding protein, named SMAR1, that also binds MARbeta. SMAR1 shares homology with SATB1 and Cux in the MAR-binding domain/Cut repeat and also with the tetramerization domain of a B cell-specific MAR-binding protein, Bright. The binding of GST-SMAR1 fusion protein to MARbeta is inhibited by the presence of an excess amount of MAR-containing DNA from the immunoglobulin kappa locus. Smar1 transcripts are most abundant in the thymus and are alternatively spliced. The smar1 gene maps to the distal portion of mouse chromosome 8 at a distance of 111.8 cM.
- Massachusetts Institute of Technology United States
- National Centre for Cell Science India
Binding Sites, DNA, Complementary, Base Sequence, Molecular Sequence Data, Chromosome Mapping, Gene Expression, Nuclear Proteins, Cell Cycle Proteins, Cell Line, DNA-Binding Proteins, Mice, Inbred C57BL, Muridae, Alternative Splicing, Mice, Genes, T-Cell Receptor beta, Animals, RNA, Nuclear Matrix, Amino Acid Sequence, Protein Binding
Binding Sites, DNA, Complementary, Base Sequence, Molecular Sequence Data, Chromosome Mapping, Gene Expression, Nuclear Proteins, Cell Cycle Proteins, Cell Line, DNA-Binding Proteins, Mice, Inbred C57BL, Muridae, Alternative Splicing, Mice, Genes, T-Cell Receptor beta, Animals, RNA, Nuclear Matrix, Amino Acid Sequence, Protein Binding
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