Supernumerary subunits NDUFA3, NDUFA5 and NDUFA12 are required for the formation of the extramembrane arm of human mitochondrial complex I
Copyright policy )Supernumerary subunits NDUFA3, NDUFA5 and NDUFA12 are required for the formation of the extramembrane arm of human mitochondrial complex I
Mammalian complex I is composed of fourteen highly conserved core subunits and additional thirty subunits acquired in the course of evolution. At present, the function of the majority of these supernumerary subunits is poorly understood. In this work, we have studied NDUFA3, NDUFA5 and NDUFA12 supernumerary subunits to gain insight into their role in CI activity and biogenesis. Using human cell lines in which the expression of these subunits was knocked down with miRNAs, we showed that they are necessary for the formation of a functional holoenzyme. Analysis of the assembly intermediates in mitochondria depleted for these subunits further suggested that they are required for assembly and/or stability of the electron transferring Q module in the peripheral arm of the CI.
Electron Transport Complex I, NADH ubiquinone oxidoreductase, NADPH Dehydrogenase, Mitochondrial complex I, NADH Dehydrogenase, Respiratory chain, Mitochondria, Protein Subunits, HEK293 Cells, Gene Knockdown Techniques, Supernumerary CI subunit, Enzyme Stability, Mitochondrial Membranes, Humans, RNA Interference, Protein Multimerization
Electron Transport Complex I, NADH ubiquinone oxidoreductase, NADPH Dehydrogenase, Mitochondrial complex I, NADH Dehydrogenase, Respiratory chain, Mitochondria, Protein Subunits, HEK293 Cells, Gene Knockdown Techniques, Supernumerary CI subunit, Enzyme Stability, Mitochondrial Membranes, Humans, RNA Interference, Protein Multimerization
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).63 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!