Supplement Figure 2. CTNNB1 knockdown in 1HAEo cells using gene silencing RNA. A) Assessment of CTNNB1 gene expression relative to GAPDH in WT 1HAEo cells treated with scrambled or CTNNB1 siRNA in the absence or presence of TGFβ1 treatment. B) Representative western blot and C) quantification of total CTNNB1 protein in scrambled siRNA or CTNNB1 siRNA-treated WT cells in the absence of presence or absence of TGFβ1 with normalization to β-actin. D) Representative western blot and E) quantification of total CTNNB1 protein in scrambled siRNA or CTNNB1 siRNA-treated in FAM13A-/- cells in the absence of presence or absence of TGFβ1 with normalization to β-actin. F) Representative western blot and G) quantification of total COL1A1 protein in scrambled siRNA or CTNNB1 siRNA-treated in FAM13A-/- cells in the absence of presence or absence of TGFβ1 with normalization to β-actin. Values were expressed as mean ± SEM (N=3 independent experiments). One-way analysis of variance with Bonferroni’s multiple comparisons test was used in panels A, C, E and G.