Three new species of the araneid spider genera Carepalxis L. Koch, 1872 and Ocrepeira Marx, 1883 from Central Brazil are described and illustrated: Carepalxis topazio new species, based on a single female from Cristalina; C. quasimodo new species, from Brasília and Jaborandi based on females and a male, this being the first male of Carepalxis described in the American continent; and Ocrepeira planalto new species, based on males and females from Cavalcante, Cristalina, Jaborandi and Alto Paraíso. A key to Carepalxis females in the New World is provided. Furthermore, the similarity between American Carepalxis and Ocrepeira is discussed, with diagnostic considerations.