cry-, pdf- and pyx-GAL4 expressing neurons are necessary for the GMF-induced geotactic positioning under the negative geotactic GMF condition. (A) The geotactic positioning of wing-injured flies. Wings were cut off under CO2 anesthesia 24 h before the tube-positioning assay. Anesthetized flies without wing injury were used as controls. (B, C, D) Comparisons of the geotactic positioning of the flies with inhibited CRY-, PDF- and Pyx-expressing neurons by the expression of TNT under the control of cry-GAL4, pdf-GAL4 and pyx-GAL4, respectively, under the negative geotactic GMF condition. Error bars: SEM. n.s., not significant. **, P