Vacuolar Degeneration of Skeletal Muscle in Transgenic Mice Overexpressing ORP150
Copyright policy )Vacuolar Degeneration of Skeletal Muscle in Transgenic Mice Overexpressing ORP150
ORP150 is a hypoxic stress-induced protein located in the endoplasmic reticulum. Transgenic mice overexpressing ORP150 (ORP-Tg) exhibit vacuolar degeneration in the heart. To determine whether vacuolization is present in skeletal muscle, we pathologically examined ORP-Tg mice. After 60 days of age, severe vacuolization was found in the soleus muscles of the hind legs of the ORP-Tg mice. Immunohistochemical staining of ORP150 revealed co-localization of ORP150 and vacuolization in the affected cells. Electron microscopy revealed a marked increase in the number of rough-surfaced endoplasmic reticula (rER) and distention of the cisterna. These findings suggest that overexpression of ORP150 causes accumulation of ORP150 in the rER, resulting in vacuolar degeneration in the skeletal muscle of ORP-Tg mice.
- National Institutes of Natural Sciences Japan
- Tottori University Japan
- Okazaki Institute for Integrative Bioscience Japan
- National Institute for Basic Biology Japan
- Institute of Laboratory Animal Science China (People's Republic of)
Muscle Cells, Proteins, Mice, Transgenic, Immunohistochemistry, Mice, Gene Expression Regulation, Muscular Diseases, Vacuoles, Animals, HSP70 Heat-Shock Proteins, Muscle, Skeletal
Muscle Cells, Proteins, Mice, Transgenic, Immunohistochemistry, Mice, Gene Expression Regulation, Muscular Diseases, Vacuoles, Animals, HSP70 Heat-Shock Proteins, Muscle, Skeletal
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