Enhancement of B-MYB Transcriptional Activity by ZPR9, a Novel Zinc Finger Protein
Copyright policy )Enhancement of B-MYB Transcriptional Activity by ZPR9, a Novel Zinc Finger Protein
By using the yeast two-hybrid system, the zinc finger protein ZPR9 was identified as one of the B-MYB interacting proteins that associates with the carboxyl-terminal conserved region of B-MYB. ZPR9 was found to form in vivo complexes with B-MYB, as demonstrated by in vivo binding assay and coimmunoprecipitation experiments of the endogenously and exogenously expressed proteins. Deletion analysis revealed that this binding was mediated by all three functional domains, an amino-terminal DNA-binding domain, a transactivation domain, and a carboxyl-terminal conserved region of B-MYB. We show that the interaction of ZPR9 with B-MYB is functional because cotransfection of ZPR9 significantly up-regulates B-MYB transcriptional activity in a dose-dependent manner. In addition, coexpression of ZPR9 with B-MYB caused the accumulation of B-MYB, as well as ZPR9, in the nucleus. Furthermore, constitutive expression of ZPR9 in human neuroblastoma cells induces apoptosis in the presence of retinoic acid. These results strongly suggest that ZPR9 plays an important role in modulation of the transactivation by B-MYB and cellular growth of neuroblastoma cells.
- Chungbuk National University Korea (Republic of)
- Pohang University of Science and Technology Korea (Republic of)
DNA, Complementary, Detergents, NECROSIS-FACTOR RECEPTOR, Apoptosis, Cell Cycle Proteins, Cell Line, C-MYB, CYTOPLASMIC DOMAIN, Genes, Reporter, HUMAN NEUROBLASTOMA-CELLS, Humans, RETINOIC ACID RECEPTOR, GENE-PRODUCT, Luciferases, LEUCINE-ZIPPER, Cell Nucleus, Microscopy, Confocal, Dose-Response Relationship, Drug, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Cell Differentiation, TRANSACTIVATION, Phosphoproteins, DNA-Binding Proteins, Microscopy, Fluorescence, A-MYB, Carrier Proteins, FACTOR FAMILY, Gene Deletion, Plasmids
DNA, Complementary, Detergents, NECROSIS-FACTOR RECEPTOR, Apoptosis, Cell Cycle Proteins, Cell Line, C-MYB, CYTOPLASMIC DOMAIN, Genes, Reporter, HUMAN NEUROBLASTOMA-CELLS, Humans, RETINOIC ACID RECEPTOR, GENE-PRODUCT, Luciferases, LEUCINE-ZIPPER, Cell Nucleus, Microscopy, Confocal, Dose-Response Relationship, Drug, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Cell Differentiation, TRANSACTIVATION, Phosphoproteins, DNA-Binding Proteins, Microscopy, Fluorescence, A-MYB, Carrier Proteins, FACTOR FAMILY, Gene Deletion, Plasmids
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