The Schizosaccharomyces pombe pla1 gene encodes a poly(A) polymerase and can functionally replace its Saccharomyces cerevisiae homologue
Copyright policy )The Schizosaccharomyces pombe pla1 gene encodes a poly(A) polymerase and can functionally replace its Saccharomyces cerevisiae homologue
We have isolated the poly(A) polymerase (PAP) encoding gene pla1 [for poly(A) polymerase] from the fission yeast Schizosaccharomyces pombe. Protein sequence alignments with other poly(A) polymerases reveal that pla1 is more closely related to Saccharomyces cerevisiae PAP than to bovine PAP. The two yeast poly(A) polymerases share significant sequence homology not only in the generally conserved N-terminal part but also in the C-terminus. Furthermore, pla1 rescues a S. cerevisiae PAP1 disruption mutant. An extract from the complemented strain is active in the specific in vitro polyadenylation assay. In contrast, recombinant PLA1 protein can not replace bovine PAP in the mammalian in vitro polyadenylation assay. These results indicate a high degree of conservation of the polyadenylation machinery among the evolutionary diverged budding and fission yeasts.
Base Sequence, Sequence Homology, Amino Acid, Genes, Fungal, Genetic Complementation Test, Molecular Sequence Data, Polynucleotide Adenylyltransferase, Pancreatitis-Associated Proteins, [SDV.GEN] Life Sciences [q-bio]/Genetics, Saccharomyces cerevisiae, Recombinant Proteins, [SDV] Life Sciences [q-bio], Fungal Proteins, Species Specificity, Schizosaccharomyces, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Escherichia coli, Amino Acid Sequence, Cloning, Molecular, RNA Processing, Post-Transcriptional, Poly A, Gene Deletion
Base Sequence, Sequence Homology, Amino Acid, Genes, Fungal, Genetic Complementation Test, Molecular Sequence Data, Polynucleotide Adenylyltransferase, Pancreatitis-Associated Proteins, [SDV.GEN] Life Sciences [q-bio]/Genetics, Saccharomyces cerevisiae, Recombinant Proteins, [SDV] Life Sciences [q-bio], Fungal Proteins, Species Specificity, Schizosaccharomyces, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Escherichia coli, Amino Acid Sequence, Cloning, Molecular, RNA Processing, Post-Transcriptional, Poly A, Gene Deletion
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