The Lawc protein is required for proper transcription by RNA polymerase II in Drosophila
Copyright policy )The Lawc protein is required for proper transcription by RNA polymerase II in Drosophila
Genetic analysis of the Drosophila leg-arista-wing complex (lawc) gene suggests a role for the Lawc protein in chromatin-related processes based on its classification as a trxG gene but the molecular mechanisms of its function remain elusive. We have found that Lawc is a small, cysteine-rich protein that is present in most of the interbands of polytene chromosomes. In agreement with this observation, Lawc co-localizes with RNA polymerase IIo (Pol IIo) and it is recruited to transcribed loci after elongation by Pol IIo has begun. Lawc interacts with the nuclear proteasome regulator dREGgamma in a yeast two-hybrid assay and both proteins co-localize on polytene chromosomes. In addition, a mutation in lawc interacts genetically with a mutation in a component of the proteasome. lawc mutants show decreased expression of some genes, while the levels of Pol IIoSer2 increase. We conclude that Lawc is required for proper transcription by RNA polymerase II in a process that involves the nuclear proteasome.
- Johns Hopkins University United States
- Emory University United States
Cell Nucleus, Male, Proteasome Endopeptidase Complex, Transcription, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Molecular Sequence Data, Chromosomes, Mammalian, Animals, Genetically Modified, Immunoenzyme Techniques, Drosophila melanogaster, Mutation, Animals, Drosophila Proteins, Female, Telomeric Repeat Binding Protein 2, Amino Acid Sequence, RNA Polymerase II, RNA, Messenger, In Situ Hybridization, Fluorescence
Cell Nucleus, Male, Proteasome Endopeptidase Complex, Transcription, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Molecular Sequence Data, Chromosomes, Mammalian, Animals, Genetically Modified, Immunoenzyme Techniques, Drosophila melanogaster, Mutation, Animals, Drosophila Proteins, Female, Telomeric Repeat Binding Protein 2, Amino Acid Sequence, RNA Polymerase II, RNA, Messenger, In Situ Hybridization, Fluorescence
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