Receptor interacting protein 140 as a thyroid hormone-dependent, negative co-regulator for the induction of cellular retinoic acid binding protein I gene
Copyright policy )Receptor interacting protein 140 as a thyroid hormone-dependent, negative co-regulator for the induction of cellular retinoic acid binding protein I gene
Over-expression of receptor interacting protein 140 (RIP140) suppressed thyroid hormone (T3) induction of cellular retinoic acid binding I protein (CRABPI) gene in P19 embryonal carcinoma cells. CRABPI induction by T3 is mediated by a direct-repeat four-element bound by T3 receptor (T3R) and retinoid receptor X (RXR). Three receptor-interacting domains (RIDs) in RIP140 mediate its interaction with T3R: one constitutive RID within the amino terminus, and two T3-dependent RIDs in the central portion and the carboxyl terminus. In co-immunoprecipitation and chromatin immunoprecipitation assays, RIP140 formed complexes with T3R/RXR in solution and on the endogenous target, the CRABPI promoter. T3 treatment resulted in elevated histone acetylation of the endogenous CRABPI gene promoter, but simultaneous expression of RIP140 resulted in significantly reduced histone acetylation of this promoter, primarily through the recruitment of HDAC4. This study presents the first evidence that over-expressed RIP140 acts as a T3-dependent negative co-regulator for T3 induction of the endogenous CRABPI gene in P19 cells.
- University of Minnesota System United States
- University of Minnesota United States
- University of Minnesota Morris United States
Receptors, Thyroid Hormone, Receptors, Retinoic Acid, Nuclear Proteins, Receptors, Tumor Necrosis Factor, Nuclear Receptor Interacting Protein 1, Repressor Proteins, Mice, Receptors, TNF-Related Apoptosis-Inducing Ligand, Gene Expression Regulation, Receptors, Estrogen, Genes, Reporter, Cell Line, Tumor, COS Cells, Animals, Triiodothyronine, Promoter Regions, Genetic, Adaptor Proteins, Signal Transducing
Receptors, Thyroid Hormone, Receptors, Retinoic Acid, Nuclear Proteins, Receptors, Tumor Necrosis Factor, Nuclear Receptor Interacting Protein 1, Repressor Proteins, Mice, Receptors, TNF-Related Apoptosis-Inducing Ligand, Gene Expression Regulation, Receptors, Estrogen, Genes, Reporter, Cell Line, Tumor, COS Cells, Animals, Triiodothyronine, Promoter Regions, Genetic, Adaptor Proteins, Signal Transducing
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