A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination
Copyright policy )A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination
Reporters of Cre and/or Flp activity are important for defining the spatial and temporal extent of Cre/Flp-mediated recombination. Here, we describe R26-CAG-LF-mTFP1, a multifunctional fluorescent reporter mouse that strongly expresses mTFP1 (bright teal fluorescent protein) after Cre- and Flp-mediated recombination. To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1. The germline excision of the Frt-flanked stop cassette in R26-CAG-LF-mTFP1 generated a Cre-dependent reporter (R26-CAG-LoxP-mTFP1). Similarly, R26-CAG-FRT-mTFP1, in which the loxP-flanked stop cassette was excised in the germline, requires only Flp to activate mTFP1 expression.
- Kyoto Prefectural University of Medicine Japan
- New York University United States
- Kyoto University Japan
Recombination, Genetic, RNA, Untranslated, Integrases, Green Fluorescent Proteins, Proteins, Mice, Transgenic, Mice, Inbred C57BL, Mice, Genes, Reporter, DNA Nucleotidyltransferases, Animals, Gene Knock-In Techniques
Recombination, Genetic, RNA, Untranslated, Integrases, Green Fluorescent Proteins, Proteins, Mice, Transgenic, Mice, Inbred C57BL, Mice, Genes, Reporter, DNA Nucleotidyltransferases, Animals, Gene Knock-In Techniques
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