TPS1Terminator Increases mRNA and Protein Yield in aSaccharomyces cerevisiaeExpression System
Copyright policy )TPS1Terminator Increases mRNA and Protein Yield in aSaccharomyces cerevisiaeExpression System
Both terminators and promoters regulate gene expression. In Saccharomyces cerevisiae, the TPS1 terminator (TPS1t), coupled to a gene encoding a fluorescent protein, produced more transgenic mRNA and protein than did similar constructs containing other terminators, such as CYC1t, TDH3t, and PGK1t. This suggests that TPS1t can be used as a general terminator in the development of metabolically engineered yeast in high-yield systems.
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Terminator Regions, Genetic, Saccharomyces cerevisiae Proteins, Cytochromes c, Saccharomyces cerevisiae, Glucosyltransferases, Gene Expression Regulation, Fungal, Protein Biosynthesis, RNA, Messenger, Transgenes, Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating), Promoter Regions, Genetic
Terminator Regions, Genetic, Saccharomyces cerevisiae Proteins, Cytochromes c, Saccharomyces cerevisiae, Glucosyltransferases, Gene Expression Regulation, Fungal, Protein Biosynthesis, RNA, Messenger, Transgenes, Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating), Promoter Regions, Genetic
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