Runx1 Expression Marks Long-Term Repopulating Hematopoietic Stem Cells in the Midgestation Mouse Embryo
Copyright policy )Runx1 Expression Marks Long-Term Repopulating Hematopoietic Stem Cells in the Midgestation Mouse Embryo
Hematopoietic stem cells (HSCs) are first found in the aorta-gonad-mesonephros region and vitelline and umbilical arteries of the midgestation mouse embryo. Runx1 (AML1), the DNA binding subunit of a core binding factor, is required for the emergence and/or subsequent function of HSCs. We show that all HSCs in the embryo express Runx1. Furthermore, HSCs in Runx1(+/-) embryos are heterogeneous and include CD45(+) cells, endothelial cells, and mesenchymal cells. Comparison with wild-type embryos showed that the distribution of HSCs among these various cell populations is sensitive to Runx1 dosage. These data provide the first morphological description of embryonic HSCs and contribute new insight into their cellular origin.
- Erasmus University Rotterdam Netherlands
- Dartmouth College United States
Immunology, Gene Dosage, Gestational Age, Embryo, Mammalian, Hematopoietic Stem Cells, Models, Biological, Immunophenotyping, DNA-Binding Proteins, Mesoderm, Mice, Inbred C57BL, Mice, Infectious Diseases, Proto-Oncogene Proteins, Core Binding Factor Alpha 2 Subunit, Immunology and Allergy, Animals, Leukocyte Common Antigens, Cell Lineage, Endothelium, Vascular, Biomarkers, Transcription Factors
Immunology, Gene Dosage, Gestational Age, Embryo, Mammalian, Hematopoietic Stem Cells, Models, Biological, Immunophenotyping, DNA-Binding Proteins, Mesoderm, Mice, Inbred C57BL, Mice, Infectious Diseases, Proto-Oncogene Proteins, Core Binding Factor Alpha 2 Subunit, Immunology and Allergy, Animals, Leukocyte Common Antigens, Cell Lineage, Endothelium, Vascular, Biomarkers, Transcription Factors
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