Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila
Copyright policy )Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila
In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNA-binding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.
- The University of Texas Southwestern Medical Center United States
Male, Ribonuclease III, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, RNA-Binding Proteins, In Vitro Techniques, Recombinant Proteins, MicroRNAs, Mutagenesis, Insertional, Adenosine Triphosphate, Drosophila melanogaster, Infertility, Animals, Drosophila Proteins, Female, RNA Interference, Amino Acid Sequence, RNA Processing, Post-Transcriptional, RNA Helicases
Male, Ribonuclease III, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, RNA-Binding Proteins, In Vitro Techniques, Recombinant Proteins, MicroRNAs, Mutagenesis, Insertional, Adenosine Triphosphate, Drosophila melanogaster, Infertility, Animals, Drosophila Proteins, Female, RNA Interference, Amino Acid Sequence, RNA Processing, Post-Transcriptional, RNA Helicases
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).286 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!