Obesity is induced in mice heterozygous for cyclooxygenase-2
Copyright policy )Obesity is induced in mice heterozygous for cyclooxygenase-2
In mice heterozygous for the cyclooxygenase-2 gene (COX-2+/-) the body weight was enhanced by 33% as compared to homozygous COX-2-/- mice. The weights of the gonadal fat pads in COX-2+/- mice were enhanced by 3.5 to 4.7 fold as compared to COX-2-/- mice and by 1.5 to 3.5 fold as compared to wild-type controls+/+ Serum leptin levels and leptin release by cultured adipose tissue of COX-2+/- mice were both elevated as compared to either control or COX-2-/- animals. The basal release of PGE2 or 6 keto PGF1alpha per fat pad over a 24 h incubation of adipose tissue was reduced by 80% and 95% respectively in tissue from COX-2-/- mice. NS-398, a specific COX-2 inhibitor, inhibited leptin release by 27% in adipose tissue from control mice, 31% in tissue from COX-1-/- mice and by 23% in tissue from COX-2+/- mice while having no effect on leptin release by adipose tissue from COX-2-/- mice. These data indicate that heterozygous COX-2 mice develop obesity which is not secondary to a defect in leptin release by adipose tissue.
- University of Tennessee System United States
- University of Tennessee Health Science Center United States
Epididymis, Leptin, Male, Mice, Knockout, Heterozygote, Cyclooxygenase 2 Inhibitors, Body Weight, Membrane Proteins, 6-Ketoprostaglandin F1 alpha, Dinoprostone, Isoenzymes, Mice, Adipose Tissue, Cyclooxygenase 2, Cyclooxygenase 1, Animals, Humans, Cyclooxygenase Inhibitors, Female, Nitrobenzenes
Epididymis, Leptin, Male, Mice, Knockout, Heterozygote, Cyclooxygenase 2 Inhibitors, Body Weight, Membrane Proteins, 6-Ketoprostaglandin F1 alpha, Dinoprostone, Isoenzymes, Mice, Adipose Tissue, Cyclooxygenase 2, Cyclooxygenase 1, Animals, Humans, Cyclooxygenase Inhibitors, Female, Nitrobenzenes
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