MicroRNA-101-5p Suppresses the Expression of the Ras-Related Protein RAP1A
Copyright policy )MicroRNA-101-5p Suppresses the Expression of the Ras-Related Protein RAP1A
MicroRNAs (miRs) are small, non-coding RNAs that negatively regulate gene expression. The stem-loop sequence miR-101-1 generates mature miR-101-5p and miR-101-3p. The function and target mRNA of miR-101-5p have not yet been elucidated in detail. Here, we demonstrate that miR-101-5p inhibits the expression of RAP1A, a member of the RAS gene family. Transfection of a miR-101-5p mimic significantly inhibited the expression of RAP1A mRNA in HeLa, HEK293, A549, and COLO201 cells. The same treatment significantly inhibited cell proliferation. The cytostatic effect with transfection of miR-101-5p was antagonized by treatment with the RAP inhibitor salirasib. These results suggested that miR-101-5p inhibits RAP1A, and thus, the expression levels of miR-101-5p regulate cell proliferation.
- Hokkeido University Japan
- Health Sciences University of Hokkaido Japan
- Hokkaido University Japan
- Hokkaido Bunkyo University Japan
460, proliferation, focal adhesion kinase (FAK), rap1 GTP-Binding Proteins, salirasib, Rac, MicroRNAs, HEK293 Cells, Cell Line, Tumor, Humans, RNA, Messenger, RNA, Small Interfering, RAS family protein, Cell Proliferation, Signal Transduction
460, proliferation, focal adhesion kinase (FAK), rap1 GTP-Binding Proteins, salirasib, Rac, MicroRNAs, HEK293 Cells, Cell Line, Tumor, Humans, RNA, Messenger, RNA, Small Interfering, RAS family protein, Cell Proliferation, Signal Transduction
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