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The EMBO Journal
Article . 2011
https://dx.doi.org/10.1184/r1/...
Other literature type . 2011
Data sources: Datacite
https://dx.doi.org/10.1184/r1/...
Other literature type . 2011
Data sources: Datacite
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Assembly of Saccharomyces cerevisiae 60S ribosomal subunits: role of factors required for 27S pre-rRNA processing.

Authors: Sahasranaman, Aarti; Dembowski, Jill; Strahler, John; Andrews, Philip; Maddock, Janine; Woolford, John L;

Assembly of Saccharomyces cerevisiae 60S ribosomal subunits: role of factors required for 27S pre-rRNA processing.

Abstract

The precise functions of most of the ∼200 assembly factors and 79 ribosomal proteins required to construct yeast ribosomes in vivo remain largely unexplored. To better understand the roles of these proteins and the mechanisms driving ribosome biogenesis, we examined in detail one step in 60S ribosomal subunit assembly-processing of 27SA(3) pre-rRNA. Six of seven assembly factors required for this step (A(3) factors) are mutually interdependent for association with preribosomes. These A(3) factors are required to recruit Rrp17, one of three exonucleases required for this processing step. In the absence of A(3) factors, four ribosomal proteins adjacent to each other, rpL17, rpL26, rpL35, and rpL37, fail to assemble, and preribosomes are turned over by Rat1. We conclude that formation of a neighbourhood in preribosomes containing the A(3) factors establishes and maintains stability of functional preribosomes containing 27S pre-rRNAs. In the absence of these assembly factors, at least one exonuclease can switch from processing to turnover of pre-rRNA.

Related Organizations
Keywords

Cell Nucleus, Exonucleases, Ribosomal Proteins, Saccharomyces cerevisiae Proteins, Molecular, Saccharomyces cerevisiae, Ribosome Subunits, Large, Eukaryotic, RNA, Ribosomal, Pre‐RRNA Processing, Gene Expression Regulation, Fungal, FOS: Biological sciences, Health Sciences, Exoribonucleases, Mutation, RNA Precursors, Cellular and Developmental Biology, Ribosome Assembly, Ribosomes, Roadblock, 69999 Biological Sciences not elsewhere classified

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
84
Top 10%
Top 10%
Top 10%
gold