Effects of IL-1 Receptor-Associated Kinase-4 Gene Silencing on Human Osteoblast-Like Cells
Copyright policy )Effects of IL-1 Receptor-Associated Kinase-4 Gene Silencing on Human Osteoblast-Like Cells
The aim of this study is to identify the effects of interleukin-1 receptor-associated kinase-4 (IRAK-4) gene silencing on human osteoblast-like cells. The siRNA sequences of the target gene, IRAK-4, were constructed and transferred into MG63 cells (control group = MG63 cells; SC group = MG63 cells transfected with scrambled IRAK-4 siRNA; KD group = MG63 cells transfected with 75 nM IRAK-4 siRNA). The morphological changes, cell growth, cell-cycle progression, apoptosis, and the expression of various cytokines and proteins were compared. Compared with the control and SC groups, IRAK-4 gene silencing in MG63 cells caused morphological changes, inhibited growth, altered the cell-cycle distribution, increased apoptosis (p < 0.05), decreased bone alkaline phosphatase and osteocalcin levels (p < 0.05), and decreased protein expression of Bcl-2/Bax and Bcl-2, p-JNK1/2, p-ERK1/2, and p-p38MAPK (p < 0.05). The results indicated that IRAK-4 gene silencing in MG63 cells inhibited cell proliferation and function and increase apoptosis, which may be related to the decreased Bcl-2/Bax ratio and inhibition of the protein expression of various components of the mitogen-activated protein kinase pathways. The results of this study may help improve the understanding of the relationship between IRAK-4 and osteoblast-like cells and the interactions between various cytokines in the periprosthetic inflammatory environment.
- Sun Yat-sen University China (People's Republic of)
Inflammation, Osteoblasts, MAP Kinase Kinase 4, MAP Kinase Signaling System, Apoptosis, MAP Kinase Kinase 7, Cell Line, Interleukin-1 Receptor-Associated Kinases, Gene Expression Regulation, Cytokines, Humans, Gene Silencing, Extracellular Signal-Regulated MAP Kinases, Cell Proliferation, bcl-2-Associated X Protein
Inflammation, Osteoblasts, MAP Kinase Kinase 4, MAP Kinase Signaling System, Apoptosis, MAP Kinase Kinase 7, Cell Line, Interleukin-1 Receptor-Associated Kinases, Gene Expression Regulation, Cytokines, Humans, Gene Silencing, Extracellular Signal-Regulated MAP Kinases, Cell Proliferation, bcl-2-Associated X Protein
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