Surfactant Protein A Blocks Recognition of Pseudomonas aeruginosa by CKAP4/P63 on Airway Epithelial Cells
Copyright policy )Surfactant Protein A Blocks Recognition of Pseudomonas aeruginosa by CKAP4/P63 on Airway Epithelial Cells
We used isogenic mutant strains that were deficient or over-expressed capsule to study the function of the alginate exopolysaccharide in the interaction of Pseudomonas aeruginosa with the human airway epithelial cells (AEC) in the presence or absence of surfactant protein A (SP-A). SP-A prevented the invasion of AEC by alginate-producing P. aeruginosa strains because of a direct effect on the AEC. Monoclonal antibodies to CKAP4/P63, the principal SP-A-binding receptor on AEC, or inhibition of its expression using specific siRNA reduced the invasion of both highly encapsulated and poorly encapsulated strains, but not the invasion of the acapsular mutant. Treatment of AEC with SP-A, monoclonal antibodies to CKAP4/P63, or CKAP4/P63-specific siRNA decreased the binding of purified alginate exopolysaccharide to AEC. Alginate binding to AEC reduced SP-A release by these cells. Because the alginate exopolysaccharide is surface-exposed, levels of SP-A may be crucial to modulate the interaction of P. aeruginosa with AEC.
- University of Southampton United Kingdom
- IE University Spain
- University of the Balearic Islands Spain
Pulmonary Surfactant-Associated Protein A, Alginates, Hexuronic Acids, Polysaccharides, Bacterial, Antibodies, Monoclonal, Membrane Proteins, Epithelial Cells, Respiratory Mucosa, Cell Line, Gene Expression Regulation, Glucuronic Acid, Pseudomonas aeruginosa, Humans, RNA Interference, RNA, Small Interfering, Protein Binding
Pulmonary Surfactant-Associated Protein A, Alginates, Hexuronic Acids, Polysaccharides, Bacterial, Antibodies, Monoclonal, Membrane Proteins, Epithelial Cells, Respiratory Mucosa, Cell Line, Gene Expression Regulation, Glucuronic Acid, Pseudomonas aeruginosa, Humans, RNA Interference, RNA, Small Interfering, Protein Binding
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