Cloning of murine TCF-1, a T cell-specific transcription factor interacting with functional motifs in the CD3-epsilon and T cell receptor alpha enhancers.
Copyright policy )Cloning of murine TCF-1, a T cell-specific transcription factor interacting with functional motifs in the CD3-epsilon and T cell receptor alpha enhancers.
CD3-epsilon gene expression is confined to the T cell lineage. We have recently identified and cloned a human transcription factor, TCF-1, that binds to a functional element in the T lymphocyte-specific enhancer of CD3-epsilon. In a panel of human cell lines, TCF-1 expression was restricted to T lineage cells. TCF-1 belonged to a novel family of genes that contain the so-called high mobility group 1 (HMG) box. Here we report the cloning of murine TCF-1. Two splice alternatives were identified that were not previously observed in human TCF-1. Murine and human TCF-1 displayed a 95.5% overall amino acid homology. Recombinant murine and human TCF-1 recognized the same sequence motif in the CD3-epsilon enhancer as judged by gel retardation and methylation interference assays. With the murine cDNA clones several aspects of TCF-1 were analyzed. First, deletion analysis revealed that a region of TCF-1 containing the HMG box was sufficient for sequence-specific binding. Second, by high stringency Northern blotting and in situ hybridization, TCF-1 expression was shown to be confined to the thymus and to the T cell areas of the spleen. Third, TCF-1 bound specifically to a functional T cell-specific element in the T cell receptor alpha (TCR-alpha) enhancer. The T lineage-specific expression and the affinity for functional motifs in the TCR-alpha and CD3-epsilon enhancers imply an important role for TCF-1 in the establishment of the mature T cell phenotype.
- Utrecht University Netherlands
- University Museum Utrecht Netherlands
Antigens, Differentiation, T-Lymphocyte, Recombination, Genetic, B-Lymphocytes, Base Sequence, CD3 Complex, T-Lymphocytes, Molecular Sequence Data, Receptors, Antigen, T-Cell, Gene Expression, DNA, Blotting, Northern, Cell Line, Mice, Enhancer Elements, Genetic, Sequence Homology, Nucleic Acid, Animals, Humans, Amino Acid Sequence, Chromosome Deletion, Transcription Factors
Antigens, Differentiation, T-Lymphocyte, Recombination, Genetic, B-Lymphocytes, Base Sequence, CD3 Complex, T-Lymphocytes, Molecular Sequence Data, Receptors, Antigen, T-Cell, Gene Expression, DNA, Blotting, Northern, Cell Line, Mice, Enhancer Elements, Genetic, Sequence Homology, Nucleic Acid, Animals, Humans, Amino Acid Sequence, Chromosome Deletion, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).151 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!