The Hlx homeobox transcription factor is required early in enteric nervous system development
Copyright policy )The Hlx homeobox transcription factor is required early in enteric nervous system development
Abstract Background Development of the enteric nervous system (ENS) requires interactions between migrating neural crest cells and the nascent gastrointestinal tract that are dependent upon genes expressed by both cell compartments. Hlx, a homeobox transcription factor gene that is expressed in mouse intestinal and hepatic mesenchyme, is required for normal embryonic growth of intestine and liver, and the Hlx-/- genotype is embryonic lethal. We hypothesized that Hlx is required for ENS development. Results Enteric neurons were identified in Hlx+/+ and Hlx-/- mouse embryos by immunostaining of embryo sections for the neural markers PGP9.5 and Phox2b, or by staining for β-galactosidase in whole-mount embryos containing the dopamine β-hydroxylase-nLacZ transgene. In Hlx+/+ embryos, neural crest cells/enteric neurons have moved from the stomach into the intestine by E10.5. By contrast, neural crest cells/enteric neurons remain largely restricted to the lateral stomach mesenchyme of Hlx-/- embryos, with only a few scattered neural crest cells/enteric neurons in the intestine between E10.5–16.5. Conclusion The Hlx homeobox transcription factor is required for early aspects of ENS development.
- University of Cincinnati United States
- St Vincent's Hospital Australia
- Cincinnati Children's Hospital Medical Center United States
- UNSW Sydney Australia
- St Vincent's Health Australia
Male, 570, Time Factors, 1.1 Normal biological development and functioning, Knockout, 610, 32 Biomedical and Clinical Sciences, Mice, Transgenic, Dopamine beta-Hydroxylase, Inbred C57BL, 3105 Genetics, Transgenic, Enteric Nervous System, Mesoderm, Mice, anzsrc-for: 32 Biomedical and Clinical Sciences, Genetics, Animals, Developmental, anzsrc-for: 31 Biological Sciences, Pediatric, Homeodomain Proteins, Mice, Knockout, Neurons, anzsrc-for: 42 Health sciences, Liver Disease, Neurosciences, Gene Expression Regulation, Developmental, Stem Cell Research, beta-Galactosidase, Immunohistochemistry, Gastrointestinal Tract, Mice, Inbred C57BL, anzsrc-for: 3105 Genetics, Gene Expression Regulation, anzsrc-for: 11 Medical and Health Sciences, Lac Operon, Neurological, anzsrc-for: 06 Biological Sciences, Congenital Structural Anomalies, Female, Digestive Diseases, 31 Biological Sciences, Research Article, Transcription Factors
Male, 570, Time Factors, 1.1 Normal biological development and functioning, Knockout, 610, 32 Biomedical and Clinical Sciences, Mice, Transgenic, Dopamine beta-Hydroxylase, Inbred C57BL, 3105 Genetics, Transgenic, Enteric Nervous System, Mesoderm, Mice, anzsrc-for: 32 Biomedical and Clinical Sciences, Genetics, Animals, Developmental, anzsrc-for: 31 Biological Sciences, Pediatric, Homeodomain Proteins, Mice, Knockout, Neurons, anzsrc-for: 42 Health sciences, Liver Disease, Neurosciences, Gene Expression Regulation, Developmental, Stem Cell Research, beta-Galactosidase, Immunohistochemistry, Gastrointestinal Tract, Mice, Inbred C57BL, anzsrc-for: 3105 Genetics, Gene Expression Regulation, anzsrc-for: 11 Medical and Health Sciences, Lac Operon, Neurological, anzsrc-for: 06 Biological Sciences, Congenital Structural Anomalies, Female, Digestive Diseases, 31 Biological Sciences, Research Article, Transcription Factors
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