A DNA Microarray-Based Genetic Screen for Nonhomologous End-Joining Mutants in Saccharomyces cerevisiae
Copyright policy )A DNA Microarray-Based Genetic Screen for Nonhomologous End-Joining Mutants in Saccharomyces cerevisiae
We describe a microarray-based screen performed by imposing different genetic selections on thousands of yeast mutants in parallel, representing most genes in the yeast genome. The presence or absence of mutants was detected by oligonucleotide arrays that hybridize to 20-nucleotide “barcodes.” We used this method to screen for components of the nonhomologous end-joining (NHEJ) pathway. Known components of the pathway were identified, as well as a gene not previously known to be involved in NHEJ, NEJ1 . Nej1 protein interacts with the amino terminus of LIF1/XRCC4, a recently recognized “guardian of the genome” against cancer.
- Johns Hopkins University United States
- Johns Hopkins University School of Medicine United States
- Johns Hopkins Medicine United States
Recombination, Genetic, Saccharomyces cerevisiae Proteins, DNA Ligases, DNA Repair, Genes, Fungal, Genetic Complementation Test, Nucleic Acid Hybridization, Saccharomyces cerevisiae, DNA-Binding Proteins, Fungal Proteins, DNA Ligase ATP, Transformation, Genetic, CCAAT-Binding Factor, Two-Hybrid System Techniques, Mutation, Oligonucleotide Array Sequence Analysis, Plasmids, Transcription Factors
Recombination, Genetic, Saccharomyces cerevisiae Proteins, DNA Ligases, DNA Repair, Genes, Fungal, Genetic Complementation Test, Nucleic Acid Hybridization, Saccharomyces cerevisiae, DNA-Binding Proteins, Fungal Proteins, DNA Ligase ATP, Transformation, Genetic, CCAAT-Binding Factor, Two-Hybrid System Techniques, Mutation, Oligonucleotide Array Sequence Analysis, Plasmids, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).133 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!