A Polyamine Metabolon Involving Aminopropyl Transferase Complexes in Arabidopsis
Copyright policy )A Polyamine Metabolon Involving Aminopropyl Transferase Complexes in Arabidopsis
The conversion of putrescine to spermidine in the biosynthetic pathway of plant polyamines is catalyzed by two closely related spermidine synthases, SPDS1 and SPDS2, in Arabidopsis. In the yeast two-hybrid system, SPDS2 was found to interact with SPDS1 and a novel protein, SPMS (spermine synthase), which is homologous with SPDS2 and SPDS1. SPMS interacts with both SPDS1 and SPDS2 in yeast and in vitro. Unlike SPDS1 and SPDS2, SPMS failed to suppress the speDelta3 deficiency of spermidine synthase in yeast. However, SPMS was able to complement the speDelta4 spermine deficiency in yeast, indicating that SPMS is a novel spermine synthase. The SPDS and SPMS proteins showed no homodimerization but formed heterodimers in vitro. Pairwise coexpression of hemagglutinin- and c-Myc epitope-labeled proteins in Arabidopsis cells confirmed the existence of coimmunoprecipitating SPDS1-SPDS2 and SDPS2-SPMS heterodimers in vivo. The epitope-labeled SPDS and SPMS proteins copurified with protein complexes ranging in size from 650 to 750 kD. Our data demonstrate the existence of a metabolon involving at least the last two steps of polyamine biosynthesis in Arabidopsis.
Binding Sites, Sequence Homology, Amino Acid, Arabidopsis Proteins, Genetic Complementation Test, Molecular Sequence Data, Arabidopsis, Saccharomyces cerevisiae, Precipitin Tests, Gene Expression Regulation, Enzymologic, Proto-Oncogene Proteins c-myc, Epitopes, Mutation, Protein Interaction Mapping, Polyamines, Putrescine, Amino Acid Sequence, Plant Lectins, Cells, Cultured, Phylogeny, Protein Binding
Binding Sites, Sequence Homology, Amino Acid, Arabidopsis Proteins, Genetic Complementation Test, Molecular Sequence Data, Arabidopsis, Saccharomyces cerevisiae, Precipitin Tests, Gene Expression Regulation, Enzymologic, Proto-Oncogene Proteins c-myc, Epitopes, Mutation, Protein Interaction Mapping, Polyamines, Putrescine, Amino Acid Sequence, Plant Lectins, Cells, Cultured, Phylogeny, Protein Binding
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