SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays
Copyright policy )SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays
A histone variant H2ABbd was recently identified, but its function is totally unknown. Here we have studied the structural and functional properties of nucleosome and nucleosomal arrays reconstituted with this histone variant. We show that H2ABbd can replace the conventional H2A in the nucleosome, but this replacement results in alterations of the nucleosomal structure. The remodeling complexes SWI/SNF and ACF are unable to mobilize the variant H2ABbd nucleosome. However, SWI/SNF was able to increase restriction enzyme access to the variant nucleosome and assist the transfer of variant H2ABbd-H2B dimer to a tetrameric histone H3-H4 particle. In addition, the p300- and Gal4-VP16-activated transcription appeared to be more efficient for H2ABbd nucleosomal arrays than for conventional H2A arrays. The intriguing mechanisms by which H2ABbd affects both nucleosome remodeling and transcription are discussed.
570, 5S, Transcription, Genetic, MESH: Trans-Activators, Chromosomal Proteins, Non-Histone, [SDV]Life Sciences [q-bio], Xenopus, DNA Footprinting, 610, [SDV.GEN] Life Sciences [q-bio]/Genetics, [INFO] Computer Science [cs], MESH: Chromosomal Proteins, Histones, Genetic, MESH: RNA, MESH: Nucleosomes, Animals, Deoxyribonuclease I, MESH: Animals, MESH: Xenopus, [INFO]Computer Science [cs], MESH: DNA Footprinting, MESH: Histones, Ribosomal, [SDV.GEN]Life Sciences [q-bio]/Genetics, MESH: Transcription, MESH: Chromatin Assembly and Disassembly, RNA, Ribosomal, 5S, Nuclear Proteins, Acetylation, MESH: Transcription Factors, Non-Histone, GENETIQUE, MESH: Deoxyribonuclease I, Chromatin Assembly and Disassembly, Nucleosomes, [SDV] Life Sciences [q-bio], Chromosomal Proteins, MESH: Dimerization, Trans-Activators, RNA, MESH: Nuclear Proteins, Transcription, Dimerization, MESH: Acetylation, Transcription Factors
570, 5S, Transcription, Genetic, MESH: Trans-Activators, Chromosomal Proteins, Non-Histone, [SDV]Life Sciences [q-bio], Xenopus, DNA Footprinting, 610, [SDV.GEN] Life Sciences [q-bio]/Genetics, [INFO] Computer Science [cs], MESH: Chromosomal Proteins, Histones, Genetic, MESH: RNA, MESH: Nucleosomes, Animals, Deoxyribonuclease I, MESH: Animals, MESH: Xenopus, [INFO]Computer Science [cs], MESH: DNA Footprinting, MESH: Histones, Ribosomal, [SDV.GEN]Life Sciences [q-bio]/Genetics, MESH: Transcription, MESH: Chromatin Assembly and Disassembly, RNA, Ribosomal, 5S, Nuclear Proteins, Acetylation, MESH: Transcription Factors, Non-Histone, GENETIQUE, MESH: Deoxyribonuclease I, Chromatin Assembly and Disassembly, Nucleosomes, [SDV] Life Sciences [q-bio], Chromosomal Proteins, MESH: Dimerization, Trans-Activators, RNA, MESH: Nuclear Proteins, Transcription, Dimerization, MESH: Acetylation, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).64 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!