PLU‐1 nuclear protein, which is upregulated in breast cancer, shows restricted expression in normal human adult tissues: A new cancer/testis antigen?
Copyright policy )PLU‐1 nuclear protein, which is upregulated in breast cancer, shows restricted expression in normal human adult tissues: A new cancer/testis antigen?
AbstractThe PLU‐1 gene is expressed at the level of message in breast cancers and breast cancer cell lines and shows restricted expression in normal adult tissues with the exception of testis. The predicted protein sequence contains several domains, including the PLU domain, which is shared by other proteins involved in transcription and/or development. We have developed a polyclonal antiserum to a C‐terminal fragment of the PLU‐1 protein, which shows little homology to other family members. Immunohistochemic analysis with the antiserum α‐PLU‐1C confirmed the nuclear localisation of PLU‐1. α‐PLU‐1C also reacted with the mouse homologue of PLU‐1 (mPlu‐1) but not with the closest family member, RBP2. Using Western blot analysis, PLU‐1 was shown to be well expressed in breast cancers and breast cancer cell lines, while it was not detected in a range of normal adult tissues. Our results suggest that the PLU‐1 protein may belong to the class of testis/cancer antigens. © 2002 Wiley‐Liss, Inc.
- Guy's and St Thomas' NHS Foundation Trust United Kingdom
- Kings College London, University of London United Kingdom
- Cancer Research UK United Kingdom
- King's College London United Kingdom
- Guy's Hospital United Kingdom
Male, 570, Jumonji Domain-Containing Histone Demethylases, Blotting, Western, Molecular Sequence Data, 610, Breast Neoplasms, Mice, Antigens, Neoplasm, Animals, Humans, Amino Acid Sequence, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Nuclear Proteins, Neoplasm Proteins, DNA-Binding Proteins, Gene Expression Regulation, Neoplastic, Repressor Proteins, Organ Specificity, COS Cells
Male, 570, Jumonji Domain-Containing Histone Demethylases, Blotting, Western, Molecular Sequence Data, 610, Breast Neoplasms, Mice, Antigens, Neoplasm, Animals, Humans, Amino Acid Sequence, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Nuclear Proteins, Neoplasm Proteins, DNA-Binding Proteins, Gene Expression Regulation, Neoplastic, Repressor Proteins, Organ Specificity, COS Cells
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