Involvement of Phosphoinositide Turnover in Ouabain Inotropism

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 1993 English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 194, pages 72-78 (issn: 0006-291X,

Authors: H, Gotoh; A, Kamiyama; R, Shibayama; M, Sawada; T, Kashimoto;

doi: 10.1006/bbrc.1993.1786

pmid: 7687441

Involvement of Phosphoinositide Turnover in Ouabain Inotropism

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Abstract

We examined whether ouabain activities phospholipases and reinforces contraction force of papillary muscles through resultant second messengers. 2-Nitro-4-carboxyphenyl-N,N-diphenylcarbamate (NCDC), the inhibitor of phospholipase C (PLC), abolished the ouabain inotropy in rabbit papillary muscles. Calphostin C, the specific inhibitor of protein kinase C (PKC), also depressed the ouabain inotropy. 12-O-Tetradecanoylphorbor-13-acetate (TPA), the specific activator of PKC, enhanced the beat-to-beat phasic contractility at low concentrations. Radioenzymatic assay revealed that ouabain treatment doubled diacylglycerol (DG) content in excised papillary muscles. We concluded that ouabain activates PLC, and the resultant second messenger, DG, augments the cardiac contraction force through activation of PKC.

Related Organizations

Iwate Medical University
Japan
Iwate University
Japan

Keywords

Dose-Response Relationship, Drug, In Vitro Techniques, Naphthalenes, Papillary Muscles, Phosphatidylinositols, Myocardial Contraction, Second Messenger Systems, Diglycerides, Enzyme Activation, Animals, Tetradecanoylphorbol Acetate, Polycyclic Compounds, Rabbits, Ouabain, Protein Kinase C

23 Research products, page 1 of 3

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