Growth Defect and Mutator Phenotypes of RecQ-Deficient Neurospora crassa Mutants Separately Result From Homologous Recombination and Nonhomologous End Joining During Repair of DNA Double-Strand Breaks
Copyright policy )Growth Defect and Mutator Phenotypes of RecQ-Deficient Neurospora crassa Mutants Separately Result From Homologous Recombination and Nonhomologous End Joining During Repair of DNA Double-Strand Breaks
Abstract RecQ helicases function in the maintenance of genome stability in many organisms. The filamentous fungus Neurospora crassa has two RecQ homologs, QDE3 and RECQ2. We found that the qde-3 recQ2 double mutant showed a severe growth defect. The growth defect was alleviated by mutation in mei-3, the homolog of yeast RAD51, which is required for homologous recombination (HR), suggesting that HR is responsible for this phenotype. We also found that the qde-3 recQ2 double mutant showed a mutator phenotype, yielding mostly deletions. This phenotype was completely suppressed by mutation of mus-52, a homolog of the human KU80 gene that is required for nonhomologous end joining (NHEJ), but was unaffected by mutation of mei-3. The high spontaneous mutation frequency in the double mutant is thus likely to be due to NHEJ acting on an elevated frequency of double-strand breaks (DSBs) and we therefore suggest that QDE3 and RECQ2 maintain chromosome stability by suppressing the formation of spontaneous DSBs.
- Saitama University Japan
Adenosine Triphosphatases, Recombination, Genetic, DNA Repair, Neurospora crassa, RecQ Helicases, DNA Helicases, Antigens, Nuclear, Chromosome Breakage, Epistasis, Genetic, DNA, DNA-Binding Proteins, Fungal Proteins, Phenotype, Suppression, Genetic, Mutation, DNA, Fungal, Ku Autoantigen, Gene Deletion
Adenosine Triphosphatases, Recombination, Genetic, DNA Repair, Neurospora crassa, RecQ Helicases, DNA Helicases, Antigens, Nuclear, Chromosome Breakage, Epistasis, Genetic, DNA, DNA-Binding Proteins, Fungal Proteins, Phenotype, Suppression, Genetic, Mutation, DNA, Fungal, Ku Autoantigen, Gene Deletion
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).16 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!